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factor m csf  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec factor m csf
    Factor M Csf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/factor+m+csf/Human+M-CSF%2C+premium+grade/10__1158_slash_2326___6066__cir___25___1602-138-32-35
    Average 96 stars, based on 97 article reviews
    factor m csf - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Bacteria:

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages
    Article Snippet: To obtain macrophages, 250.000 monocytes were cultured in RPMI 1640 medium (#23400-021, Gibco, USA) for 2 h in 24-well plates. .. After this, nonadherent cells were removed by three washes with 37 °C pre-warmed PBS, and the medium was replaced with complete culture medium (RPMI 1640 medium containing 10% fetal bovine serum (Natocor, Argentina), 2 mM L-glutamine (#35050061, Gibco), 100 U/ml penicillin and 0.1 mg/ml streptomycin (#15240-062, Gibco), 10 ng/ml macrophage colony-stimulating factor (M-CSF) (#130-096-489, Miltenyi Biotec) alone or plus HKBa (1 × 10 8 bacteria/ml), or its antigens: L-Omp19 [500 ng/ml], U-Omp19 [500 ng/ml], BA-LPS [1000 ng/ml], RNA [5 μg/ml], and DNA [5 μg/ml], all from B. abortus , which were cultured for 5 days at 37 °C and 5% CO 2 . .. Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA).

    Cell Culture:

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages
    Article Snippet: To obtain macrophages, 250.000 monocytes were cultured in RPMI 1640 medium (#23400-021, Gibco, USA) for 2 h in 24-well plates. .. After this, nonadherent cells were removed by three washes with 37 °C pre-warmed PBS, and the medium was replaced with complete culture medium (RPMI 1640 medium containing 10% fetal bovine serum (Natocor, Argentina), 2 mM L-glutamine (#35050061, Gibco), 100 U/ml penicillin and 0.1 mg/ml streptomycin (#15240-062, Gibco), 10 ng/ml macrophage colony-stimulating factor (M-CSF) (#130-096-489, Miltenyi Biotec) alone or plus HKBa (1 × 10 8 bacteria/ml), or its antigens: L-Omp19 [500 ng/ml], U-Omp19 [500 ng/ml], BA-LPS [1000 ng/ml], RNA [5 μg/ml], and DNA [5 μg/ml], all from B. abortus , which were cultured for 5 days at 37 °C and 5% CO 2 . .. Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA).

    Article Title: Multinucleated giant cells in human pancreatic cancer are a distinct macrophage population undergoing a DNA damage response and associated with an aggressive tumor microenvironment
    Article Snippet: Monocytes were isolated using Pan Monocyte Isolation Kit, human (Miltenyi Biotec, 130-096-537) and plated at a density of 3 × 106 cells per well in 6- well plates. .. Cells were cultured in RPMI (ECB9006L, Euroclone) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (97068-085, VWR), 1% penicillin–streptomycin (ECB3001D, Euroclone), and 1% L-glutamine (BCP04-80100, Bio-cell), together with 50 ng/mL macrophage colony-stimulating factor (M-CSF) (130-096-492, Miltenyi) for 5 days to promote differentiation into macrophages. ..

    other:

    Article Title: Recognition of Brucella abortus drives M2 like polarization and impaired antigen presentation in monocyte derived macrophages.
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats of healthy anonymous blood donors via density gradient centrifugation using Ficoll‒Hypaque (#171440-03, GE Healthcare, USA).

    Article Title: IGF2BP2 Deficiency in Macrophages Impairs Migration, Reprograms Metabolism, and Limits Tumor Progression
    Article Snippet: The remaining cells were resuspended in a standard medium supplemented with macrophage colony-stimulating factor (M-CSF, 50 ng/ml, #130-101-704, Miltenyi Biotec Bergisch Gladbach, Germany) and transferred to a 75 cm2 culture flask overnight for initial attachment.

    Isolation:

    Article Title: Herring roe PLs promote SPM biosynthesis in macrophages and a keratinocyte/fibroblast coculture as a model of psoriasis
    Article Snippet: .. Peripheral blood mononuclear cells were isolated from buffy coats by LymphoprepTM (AXIS-SHIELD PoC AS, 1114547) density gradient, and monocytes were selected by plastic adherence and differentiated into MDMs by culture in AIM V medium (Gibco, 12055083) with 5% CTSTM Immune Cell Serum Replacement and 10 ng/ml macrophage colony-stimulating factor (M-CSF) (Miltenyi Biotec, 130-093-963) for 5 days, followed by incubation without M-CSF. .. MDMs from each donor were treated in 1.5 ml per well in a 6-well plate (3 wells per condition) with indicated final percentages (w/w) of HRO or PEHeRo, by dilution of the prepared emulsions in phenol- and serum-free CTSTM AIM-VTM Medium (Gibco, A3830801) supplemented with penicillin-streptomycin.

    Incubation:

    Article Title: Herring roe PLs promote SPM biosynthesis in macrophages and a keratinocyte/fibroblast coculture as a model of psoriasis
    Article Snippet: .. Peripheral blood mononuclear cells were isolated from buffy coats by LymphoprepTM (AXIS-SHIELD PoC AS, 1114547) density gradient, and monocytes were selected by plastic adherence and differentiated into MDMs by culture in AIM V medium (Gibco, 12055083) with 5% CTSTM Immune Cell Serum Replacement and 10 ng/ml macrophage colony-stimulating factor (M-CSF) (Miltenyi Biotec, 130-093-963) for 5 days, followed by incubation without M-CSF. .. MDMs from each donor were treated in 1.5 ml per well in a 6-well plate (3 wells per condition) with indicated final percentages (w/w) of HRO or PEHeRo, by dilution of the prepared emulsions in phenol- and serum-free CTSTM AIM-VTM Medium (Gibco, A3830801) supplemented with penicillin-streptomycin.



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